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KMID : 0350519950480041173
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Journal of Catholic Medical College
1995 Volume.48 No. 4 p.1173 ~ p.1186
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Loss of Heterozygosity Affecting the APC and p53 Tumor Suppressor Gene Loci in Colorectal Cancers and Its Prognostic Significance
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Abstract
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Development of the human colorectal cancer is associated with several distinct genetic abnormalities involving both dominant-acting oncogenes (K-ras, c-src) and tumor suppressor genes (APC, DCC, p53) which undergo inactivation or loss. In
colorectal
tumors, the common molecular alteration is localized in the 17p13 and 5q21 loci encoding p53 and APC (Adenomatous polyposis coli)gene, respectively. The identification of these genes may help the understanding of the pathogenesis of colorectal
neoplasia.
In order to determine whether the frequency of the genetic alterations varies with sex, age, tumor size, site, pathologic parameters such as degree of differentiation, tumor stage, mucin component, lymphoid reaction, tumor invasion pattern, vein
and
nerve invasion, lymph node metastasis, and another parameters, such as disease-free survival, distant metastasis and patient outcome, the auther analyzed the loss of heterozygosity (LOH) of apc AND P53 genes in paraffin-embedded specimens of 48
colorectal cancers oy use of the polymerase chain reaction and restriction fragment length polymorphism.
@ES The results were as follows :
@EN 1) LOH affecting APC was found in 15 out of 31 (48.4%) heterozygous patients, while LOH of P53 locus was observed in 11 out of 26 (42.3%) patients.
2) Among 48 patients, LOH at both APC and p53 loci was observed in 5 (10.4%).
3) No statistically significant associations were found between LOH of APC gene and the proposed parameters.
4) Relationship between LOH of p53 and histologic differentiation, lymphoid reaction was significant (P< 0.05) but survival was not correlated.
5) Statistically significant associations were found between overall survival of the colorectal concer patients and distant metastasis, Astler-Coller stage, lymphoid reaction, invasion pattern, nerve invasion, vein invasion, lymph node
metastasis,
and
disease free survival.
Above results suggest that LOH of APC nor that of p53 have significant association with survival of the colorectal cancer patients.
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KEYWORD
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